质粒类型：启动子报告载体

启动子：SV40

载体大小：5010 bp 

5' 测序引物及序列：RVprimer3 Fwd:CTAGCAAAATAGGCTGTCCC

载体抗性：Ampicillin (氨苄青霉素)

The pGL3 Luciferase Reporter Vectors provide a basis for the quantitative analysis of factors that potentially regulate mammalian gene expression. These factors may be cis-acting, such as promoters and enhancers, or trans-acting, such as various DNA-binding factors. The backbone of the pGL2 Luciferase Reporter Vectors was redesigned for the pGL3 Vectors for increased expression, and contains a modified coding region for firefly (Photinus pyralis)luciferase that has been optimized for monitoring transcriptional activity in transfected eukaryotic cells. The assay of this genetic reporter is rapid, sensitive and quantitative. In addition, the Luciferase Reporter Vectors contain numerous features aiding in the structural characterization of the putative regulatory sequences under investigation.

The pGL3-Promoter Vector contains an SV40 promoter upstream of the luciferase gene. DNA fragments containing putative enhancer elements can be inserted either upstream or downstream of the promoter-luc+ transcriptional unit.

pGL3-promoter(点击下载)

pGL3-promoter质粒序列.txt