pSELECT-GFP-LC3 is a mammalian expression vector containing the human LC3B gene fused at its 5’ end to the green fluorescent protein (GFP) gene. This plasmid is selectable in bacteria and mammalian cells using Zeocin?. Expression of the GFP-LC3B fusion gene allows to visualize autophagosome formation in real time in live cells. During autophagosome formation, GFP-LC3B is processed and recruited to the autophagosome membrane, where it can be imaged as cytoplasmic puncta by high resolution fluorescence microscopy. The percentage of GFP-LC3B positive  cells can be determined and is indicative of autophagosome formation.

GFP::LC3B fusion gene was generated by fusing a GFP variant to the 5’ end of the human LC3B gene. A synthetic intron was added between both moieties to increase the activity of GFP. This hybrid protein absorbs blue light (major peak at 480 nm) and emits green light (major peak at 505 nm).

Plasmid construct has been confirmed by restriction analysis and sequencing.
Plasmid DNA was purified by ion exchange chromatography and lyophilized.

pSELECT-GFP-LC3 载体说明(点击下载)

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