质粒类型：大肠杆菌表达载体

克隆方法：多克隆位点，限制性内切酶

载体大小：7412bp 

5' 测序引物及序列：T7

载体标签：Intein

载体抗性：Ampicillin (氨苄青霉素)

pTYB11 is an E. coli cloning and expression vector (7414 bp) used in the IMPACT Protein Purification System which allows the overexpression of a target protein as a fusion to a self-cleavable affinity tag. It is a N-terminal fusion vector designed for in-frame insertion of a target gene into the polylinker, downstream of the intein tag (the Sce VMA intein/chitin binding domain, 55 kDa). This allows the N-terminus of the target protein to be fused to the intein tag. The self-cleavage activity of the intein allows the release of the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein. This vector can be used for expression and purification of a native target protein without any vector-derived residues.

pTYB11 载体说明(点击下载)

pTYB11质粒序列.txt

pTYB11 载体序列(点击下载)