产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VDN0207 | BLR(DE3) | Novagen |
配套大肠杆菌
|
询价 | 现货 |
出品公司:Novagen
菌株类型:E.coli
培养基:LB培养基
生长条件:37℃,有氧
基因型:F-ompT hsdSB(rB- mB-) gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR)
抗性:四环素
质粒转化条件:42℃ 热激
应用:蛋白表达
诱导方法:IPTG
BLR is a recA- derivative of BL21 that improves plasmid monomer yields and may help stabilize target plasmids containing repetitive sequences or whose products may cause the loss of the DE3 prophage (1, 2).
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) Δ(srl-recA)306::Tn10 (TetR)