载体用途：酵母表达载体；酿酒酵母表达载体

质粒大小：5726bp

原核抗性：Amp+

筛选标记：URA3

启动子：URA3，T7，T3

复制子：2μFI ori

克隆菌株：DH5a

培养条件：37℃

表达宿主：酵母细胞

表达诱导方式：半乳糖诱导

5'测序引物：M13-R（5'-CAGGAAACAGCTATGACC-3'）

3'测序引物：根据序列设计引物

The order of the major features in this plasmid is:ori(f1) - lacZ - T7 promoter - MCS (KpnI-SacI) - T3 promoter - lacI - ori(pMB1) - ampR - ori (2 micron) - URA3. Contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.4 + or - 1.4% of progeny per doubling. YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. Can be used to produce ssDNA. Contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. Restriction digests of the clone give the following sizes (kb): BamHI--5.8; EcoRI--5.8; PstI--4.0, 1.7; PvuII--5.2, 0.54. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 416322 Hosts: E.coli DH5alpha, Saccharomyces cerevisiae, E.coli.

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pRS426质粒序列.txt