载体用途：基因敲除载体

复制子：pSC101

载体大小：9332 bp

原核抗性：Amp+、Chl+（建议使用双抗性）

克隆菌株：DH5a

培养温度：30℃

质粒拷贝数（Copy number）：低

5' 测序引物：FLP-R（5'-GAGCTTTGTTGTAGGTGGACC-3'）

3' 测序引物：FLP-F（5'-TTGATGCGCTGGCAGTGTTC-3'）

pCP20 is an ampicillin and Cm^R plasmid that shows temperature-sensitive replication and thermal induction of FLP synthesis. Cm^R and Km^R mutants were transformed with pCP20, and ampicillin-resistant transformants were selected at 30℃, after which a few were colony-purified once nonselectively at 43℃ and then tested for loss of all antibiotic resistances. The majority lost the FRT-flanked resistance gene and the FLP helper plasmid simultaneously. 

pCP20含有DNA重组酶基因（FLP），其主要作用是消除基因敲除留下的疤痕。

pCP20产品说明书.pdf

pCP20质粒序列.txt