质粒类型：逆转录病毒载体

高拷贝/低拷贝：低拷贝

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：6132 bp

5' 测序引物及序列：pLNCX-F:AGCTGGTTTAGTGAACCGTCAGATC

3' 测序引物及序列：pLNCX-R:ACCTACAGGTGGGGTCTTTCATTCCC

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Neomycin (新霉素)

pLNCX2 contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV), and is designed for retroviral gene delivery and expression (1– 3). Upon transfection into a packaging cell line, pLNCX2 can transiently express, or integrate and stably express, a transcript containing Y+ (the extended viral packaging signal) a selectable marker, and the gene of interest. The 5' viral LTR in this vector contains viral promoter/enhancer sequences that control expression of the neomycin resistance (Neor) gene for antibiotic selection in eukaryotic cells. A gene of interest can be cloned into the multiple cloning site immediately downstream of the human cytomegalovirus (CMV) immediate early promoter (PCMV). pLNCX2 also includes the Col E1 origin of replication and E. coli Ampr gene for propagation and antibiotic selection in bacteria.

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pLNCX2质粒序列.txt

pGEX-4T-2 载体序列(点击下载)