质粒类型：哺乳动物双杂交系统

高拷贝/低拷贝：高拷贝

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：7400 bp

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Neomycin (新霉素)

The CheckMate™ Mammalian Two-Hybrid System provides positive control vectors that encode and express two proteins known to interact in vivo (13). MyoD is expressed in skeletal muscle and is a myogenic regulatory protein (14,15). The Id protein acts as a negative regulator of myogenic differentiation (16). MyoD and Id are members of the helix-loop-helix family of nuclear proteins. The pBIND-Id Control Vector contains a segment of murine Id cDNA (amino acids 29–148; 13,16) cloned in-frame with the GAL4 DNA-binding domain of the pBIND Vector (Figure 6). The pACT-MyoD Control Vector contains a segment of murine MyoD cDNA (amino acids 1–318; 13,15) cloned in-frame with the VP16 activation domain of the pACT Vector (Figure 6).

pBIND 载体说明(点击下载)

pACT 载体说明(点击下载