质粒类型：RNAi载体

启动子：H1

载体大小：2795 bp 

5' 测序引物及序列：M13F(-40):GTTTTCCCAGTCACGAC

载体抗性：Ampicillin (氨苄青霉素)

The pSilencer siRNA Expression Vectors are linearized with both BamH 1 and Hind III to facilitate directional cloning. They are purified to remove the digested insert so that it cannot re-ligate with the vector. This greatly increases the percentage of clones bearing the hairpin siRNA-coding insert after ligation, reducing the time and effort required to screen clones. Both pSilencer 2.0-U6 and pSilencer 3.0-H1 are linearized with the same restriction enzymes, so that a given hairpin siRNA insert can be subcloned into either vector using the 5' overhangs left by restriction enzyme digestion.

pSilencer 3.0-H1 载体说明(点击下载)

pSilencer 3.0-H1质粒序列.txt