| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VRC0345 | RNAi-Ready pSIREN-RetroQ | Clontech |
RNAi载体
|
询价 | 现货 |
质粒类型：RNAi载体
高拷贝/低拷贝：低拷贝
启动子：Human U6
克隆方法：多克隆位点，限制性内切酶
载体大小：6455bp
5' 测序引物及序列：U6-F: ATGGACTATCATATGCTTACCGTA (Clontech )
载体抗性：Ampicillin (氨苄青霉素)
筛选标记：Puromycin (嘌呤霉素)
RNAi-Ready pSIREN-RetroQ is a self-inactivating retroviral expression vector designed to express a small hairpin RNA (shRNA) using the human U6 promoter (PU6; RNA Pol III-dependent). RNAi-Ready pSIREN-RetroQ is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an oligonuceotide encoding an appropriate shRNA is ligated into the vector. You can transfect your pSIREN-RetroQ construct as a plasmid expression vector, or—upon transfection into a packaging cell line—this vector can transiently express, or integrate and stably express a viral genomic transcript containing the human U6 promoter and the shRNA. The vector contains a puromycin resistance gene for the selection of stable transfectants.
