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pTet-On产品信息
订购信息
产品货号 载体名称 出品公司 载体用途 产品价格 出货周期
HG-VSC0460 pTet-On Clontech
四环素调控系统
询价 现货
产品参数

质粒类型:四环素调控系统

启动子:CMV

克隆方法:多克隆位点,限制性内切酶

载体大小:7400 bp 

载体抗性:Ampicillin (氨苄青霉素)

筛选标记:Neomycin (新霉素)

载体介绍

pTet-On expresses the reverse tet-responsive transcriptional activator (rtTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-On is similar to pTet-Off except for four amino acid changes that convert the TetR to a rTetR, and consequently, tTA to rtTA. (pTet-Off also contains several silent mutations.) pTet-On was originally described as pUHD17-1neo by Gossen et al. pTet-On can be distinguished from pTet-Off by digestion with Hind III.

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载体序列
质粒图谱

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