| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VSC0462 | pTet-Off | Clontech |
四环素调控系统
|
询价 | 现货 |
质粒类型：四环素调控系统
启动子：CMV
克隆方法：多克隆位点，限制性内切酶
载体大小：7391 bp
载体抗性：Ampicillin (氨苄青霉素)
筛选标记：Neomycin (新霉素)
pTet-Off expresses the tet-responsive transcriptional activator (tTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-Off was originally described as pUHD15-1neo by Resnitzky et al. (1994) and was created by insertion of a neomycin resistance gene into pUHD15-1 (Gossen & Bujard, 1992). pTet-Off can be distinguished from pTet-On by digestion with Hind III.
