载体类型：腺相关病毒系统

表达水平：高

启动子：CMV

载体大小：6.1 kb

3‘ 端测序引物：Beta-globin-F:ATTCTGAGTCCAAGCTAGGC

5‘ 端测序引物：hGH-PA-R:CCAGCTTGGTTCCCAATAGA

载体抗性：Ampicillin (氨苄青霉素)

The vector pAAV-IRES-hrGFP contains a dicistronic expression cassette in which the Renilla reniformis green fluorescent protein (hrGFP) gene is expressed as a second open reading frame downstream of the encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES). The hrGFP gene serves as a useful marker for titering the virus and ascertaining the infection efficiency for the desired target cell type. Another unique feature of this vector is the FLAG sequence (3x) at the c-terminal of MCS. The foreign gene could be cloned in frame with the tag and the protein could be further purified or detected by anti-FLAG antibody.

pAAV-IRES-hrGFP 载体说明(点击下载)