质粒类型：大肠杆菌表达载体

表达水平：高

启动子：Tac promoter

克隆方法：多克隆位点，限制性内切酶

载体大小：4972 bp

5' 测序引物：pGEX5'（5'-GGGCTGGCAAGCCACGTTTGGTG-3'） 

3' 测序引物：pGEX3'（5'-CCGGGAGCTGCATGTGTCAGAGG-3'）

载体标签：N-GST

载体抗性：Ampicillin (氨苄青霉素)

There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use GST SpinTrap™ microspin columns or GST MultiTrap™ 4B 96-well plates. For sample volumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 ml, use LabMate™ reservoir together with GST GraviTrap. All formats described can be used for preparation of samples in parallel. In addition GST HiTrap™ 1 and 5 ml columns and GST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design system. Alternatively, Gluthatione Sepharose bulk media are available from 10 ml up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium and 5 empty gravity flow columns with required buffers. For simplified buffer preparation use the GST Buffer Kit. Ordering information for all associated products is listed below.

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pGEX-5X-1质粒序列.txt