产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VYN0155 | pET-17b(+) | Novagen |
原核表达载体
|
询价 | 现货 |
载体别名:pET17b, pet 17b
质粒类型:大肠杆菌表达载体
表达水平:高
克隆方法:多克隆位点,限制性内切酶
载体大小:3306bp
5' 测序引物:T7
3' 测序引物:T7-ter
载体标签:N-T7
载体抗性:Ampicillin (氨苄青霉素)
The pET-17b vector carries an N-terminal 11aa T7 tag sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker. Unique sites (except for the two BstX I sites) are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circluar map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.