| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VYN0196 | pRSFDuet-1 | Novagen |
原核表达载体
|
询价 | 现货 |
质粒类型：大肠杆菌表达载体（双启动子）
表达水平：高
克隆方法：多克隆位点，限制性内切酶
载体大小：3829 bp
5' 测序引物：T7
3' 测序引物：T7-ter
载体标签：N-His, C-S
载体抗性：Kanamycin (卡那霉素)
备注：pRSFDuet-1 carries the RSF1030 replicon, lacI gene and kanamycin resistance gene.
pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the RSF1030-derived RSF replicon, lacI gene and kanamycin resistance gene (KnR). This vector can be used in combination with pACYCDuet™-1, pCDFDuet™-1, and pETDuet™-1 in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. Unique sites are shown on the circle map.
