质粒名称：pCOLAduet-1，pCOLAduet1，pCOLAduet 1

质粒类型：原核表达载体（双启动子）；大肠杆菌表达载体（双启动子）

表达水平：高

克隆方法：多克隆位点，限制性内切酶

载体大小：3719 bp

5' 测序引物：T7（5'-TAATACGACTCACTATAGGG-3'）

3' 测序引物：T7-ter（5'-TGCTAGTTATTGCTCAGCGG-3'）

载体标签：N-His, C-S

载体抗性：Kanamycin (卡那霉素)

pCOLADuet-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His•Tag sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S•Tag peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to 8 target genes.

pCOLAduet-1产品说明书.pdf

pCOLAduet-1质粒序列.txt