质粒类型：大肠杆菌表达载体

表达水平：高

启动子：Tac promoter

克隆方法：多克隆位点，限制性内切酶

载体大小：5752 bp

5' 测序引物：MalE-f（5'-GGTCGTCAGACTGTCGATGAAGCC-3'）

3' 测序引物：M13-f（5'-TGTAAAACGACGGCCAGT-3'）

载体标签：N-MBP, N-Factor Xa

载体抗性：Ampicillin (氨苄青霉素)

备注：融合表达麦芽糖结合蛋白MBP，蛋白定位于细胞周质。

The vector pMAL-p5X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa.

MBP fusions made with this vector include an N-terminal signal sequence, so the fusion protein is directed to the periplasm. The MBP has been engineered for tighter binding to amylose resin.

A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding maltose-binding protein). The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest.

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pMAL-p5X质粒序列.txt