载体用途：酵母表达载体（整合型）；酿酒酵母表达载体

质粒大小：4456bp

原核抗性：Amp+

筛选标记：HIS3

复制子：pUC ori

克隆菌株：DH5a

培养条件：37℃

表达宿主：YPH499、YPH500等酿酒酵母

5'测序引物：T7（5'-TAATACGACTCACTATAGGG-3'）

3'测序引物：M13-R（5'-CAGGAAACAGCTATGACC-3'）

pRS4xx系列质粒被用于基因的穿梭载体克隆酿酒酵母，但能够在大肠杆菌中复制。虽然在大肠杆菌中，他们从pMB1的复制原点复制至pBR322。他们携带的bla（AmpR）标记用于选择用氨苄青霉素和复制的噬菌体F1原点单链DNA 。

pRS403 through pRS406 are integrative plasmids (YIp); pRS413through pRS416 (shown below) are centromeric plasmids (YCp),and differ from the YIp by the insertion of a centromere (CEN)and autonomously replicating sequence (ARS); pRS423 throughpRS426 are episomal plasmids (YEp), and carry instead theorigin of replication from the yeast 2µ circle plasmid along withREP3 and FRT sequences. The 4 vectors in each series differonly in the yeast auxotrophic marker used: HIS3 (pRS4x3), TRP1(pRS4x4), LEU2 (pRS4x5) or URA3 (pRS4x6).

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