质粒类型：慢病毒载体

高拷贝/低拷贝：低拷贝

启动子：TET

克隆方法：多克隆位点，限制性内切酶

载体大小：8226 bp 

5' 测序引物及序列：EGFP-C Forward:CATGGTCCTGCTGGAGTTCGTG

载体标签：GFP

载体抗性：Ampicillin (氨苄青霉素)

TET promoter (minimal CMV promoter containing seven upstream Tet-operator sites) transcribes GFP and miR30-based shRNA targeting FF3. The FF3 hairpin can be replaced with any other hairpin by cloning into the EcoR1/Xho1 site.

Please note that the full plasmid sequence shown here is for the empty vector only and does not contain the FF3 targeting sequence (AGCTCCCGTGAATTGGAATCCTAGTGAAGCCACAGATGTAGGATTCCAATTCAGCGGGAGCC).

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pPRIME-TET-GFP-FF3质粒序列.txt