产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VSC0890 | pTet-Off Advanced | Clontech |
四环素调控系统
|
询价 | 现货 |
质粒类型:四环素调控系统
启动子:CMV
克隆方法:多克隆位点,限制性内切酶
载体大小:7140 bp
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:Neomycin (新霉素)
pTet-Off-Advanced expresses an improved version of the Tet (tetracycline)-controlled transactivator protein called tTA-Advanced (1–4). It is expressed to higher levels than the original tTA used in the Tet-Off® System (2). The tTA-Advanced protein is a fusion of amino acids 1–207 of the Tet repressor (TetR) and 39 amino acids containing three minimal "F"-type transcriptional activation domains (ADs) from the VP16 protein of herpes simplex virus. The gene is fully synthetic, lacks cryptic splice sites in the mRNA, and utilizes human codon preferences for stable expression in mammalian cells.
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