质粒类型：RNAi载体

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：6470 bp 

5' 测序引物：Luc-F, M13-F20

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Puromycin (嘌呤霉素)

The Ambion® pMIR-REPORT™ miRNA Expression Reporter Vector System is for the measurement of miRNA expression in cells. It contains two mammalian expression vectors—one for cloning miRNA targets and evaluating miRNA regulation, and the other for the normalization of transfection efficiency.

• Clone miRNA targets and evaluate miRNA regulation

• Screen putative miRNA target sequences

• Includes both reporter and control vectors

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression. This validated reporter system contains two mammalian expression vectors. The pMIR-REPORT™ Luciferase miRNA Expression Reporter Vector contains firefly luciferase under the control of a mammalian promoter/terminator system, with an miRNA target cloning region downstream of the luciferase translation sequence. This vector is optimized for cloning miRNA targets and evaluating miRNA regulation. A second vector, pMIR-REPORT™ Beta-galactosidase Reporter Control Vector, is provided for normalizing transfection efficiency.

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pMIR-REPORT Luciferase质粒序列.txt