质粒类型：Gateway系统

克隆方法：Gateway

载体大小：4291bp 

5' 测序引物及序列：M13F

3' 测序引物及序列：M13R

载体抗性：Zeocin

备注：载体本身含有ccdB致死基因，能够使得普通的DH5a等细菌死亡，必须使用DB3.1感受态细胞进行质粒扩增。用来构建Gateway系统入门载体。

Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR product is directionally cloned with efficiencies typically >99%. The pDONR™/Zeo vector haa a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.

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