细胞生长：贴壁生长
细胞形态：成纤维细胞样
细胞数量：1×10⁶个
细胞传代：1:3传代；2~3天换液1次
细胞特性：Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. Fibroblasts within tissues are exposed to a dynamic mechnical environment, which influences the structure integrity of both healthy and healing soft tissue. Fibroblasts secrete a nonrigid extracellular matrix that is rich in type I and/or type III collagen. Besides, it has been reported that the growth of splenic fibroblasts has been increased in lymphoproliferatived diseases.
MLF (Mouse Lymphatic Fibroblasts) from Yrbio are isolated from mouse lymph nodes. MLF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MLF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. MLF are negative mycoplasma, bacteria, yeast and fungi. MLF are guaranteed to further expand for 10 population doublings at the condition provided by Yrbio.
细胞纯度：93％
细胞活力：87％（Viability by Trypan Blue Exclusion）
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌
细胞冻存：液氮冻存（基础培养基+10%DMSO+20%FBS）
细胞运输：干冰运输（1 Vial）或活细胞运输（T-25 flasks）
细胞用途：只可用于科研，不可用于临床诊断和治疗

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成纤维细胞培养基（Fibroblast Medium）
气相：空气，95%；二氧化碳，5%
温度：37摄氏度