LOCUS Exported File 10400 bp ds-DNA circular SYN 20-12月-2017 DEFINITION . ACCESSION . VERSION . KEYWORDS pLVX-AcGFP-N1-Fluc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10400) AUTHORS . TITLE Direct Submission JOURNAL Exported by HonorGene（奥诺基因）：http://www.honorgene.com FEATURES Location/Qualifiers source 1..10400 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /note="3' LTR" /note="color: #ffe4c4" misc_feature 681..806 /note="HIV-1 Psi" /note="color: #ffe4c4" misc_feature 1303..1536 /note="RRE" /note="color: #ffe4c4" misc_feature 2028..2143 /note="cPPT/CTS" /note="color: #ffe4c4" enhancer 2201..2504 /note="CMV enhancer" /note="color: #ffffff" promoter 2505..2708 /note="CMV promoter" /note="color: #ffffff; direction: RIGHT" CDS 2823..4472 /codon_start=1 /note="Fluc" /note="color: #ffcc00" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" CDS 4488..5207 /codon_start=1 /note="AcGFP1" /note="color: #05fd14" /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF GFVTAAAITHGMDELYK" promoter 5235..5734 /note="PGK promoter" /note="color: #ffffff; direction: RIGHT" CDS 5755..6354 /codon_start=1 /note="PuroR" /note="color: #ccffcc" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" misc_feature 6368..6956 /note="WPRE" /note="color: #ffffff" CDS complement(6839..6850) /codon_start=1 /note="Factor Xa site" /note="color: #cc99b2 Cleavage site after base 6838" /translation="IEGR" LTR 7163..7796 /note="3' LTR" /note="color: #ffe4c4" protein_bind 7949..7965 /note="lac operator" /note="color: #31849b" promoter complement(7973..8003) /note="lac promoter" /note="This reverse directional feature has 3 segments: ???1:?7973?..?7979?/?#ffffff?/?-10 ???2:?7980?..?7997?/?#ffffff ???3:?7998?..?8003?/?#ffffff?/?-35" protein_bind 8018..8039 /note="CAP binding site" /note="color: #31849b" rep_origin complement(8327..8915) /direction=LEFT /note="ori" /note="color: #ffff00" CDS complement(9086..9946) /codon_start=1 /note="AmpR" /note="This feature has 2 segments: ???1:?9086?..?9877?/?#ccffcc ???2:?9878?..?9946?/?#ccffcc?/?signal?sequence Cleavage site after base 9877" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(9947..10051) /note="AmpR promoter" /note="color: #ffffff; direction: LEFT" polyA_signal 10099..10233 /note="SV40 poly(A) signal" /note="color: #a6acb3" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg 2881 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa 2941 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt 3001 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg 3061 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag 3121 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca 3181 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc 3241 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg 3301 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg 3361 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg 3421 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct 3481 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg 3541 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat 3601 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt 3661 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc 3721 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct 3781 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat 3841 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg 3901 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga 3961 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg 4021 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt 4081 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt 4141 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc 4201 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac 4261 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg 4321 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg 4381 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca 4441 taaaggccaa gaagggcgga aagatcgccg tgcgggatcc accggtcatg gtgagcaagg 4501 gcgccgagct gttcaccggc atcgtgccca tcctgatcga gctgaatggc gatgtgaatg 4561 gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 4621 tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg gtgaccaccc 4681 tgagctacgg cgtgcagtgc ttctcacgct accccgatca catgaagcag cacgacttct 4741 tcaagagcgc catgcctgag ggctacatcc aggagcgcac catcttcttc gaggatgacg 4801 gcaactacaa gtcgcgcgcc gaggtgaagt tcgagggcga taccctggtg aatcgcatcg 4861 agctgaccgg caccgatttc aaggaggatg gcaacatcct gggcaataag atggagtaca 4921 actacaacgc ccacaatgtg tacatcatga ccgacaaggc caagaatggc atcaaggtga 4981 acttcaagat ccgccacaac atcgaggatg gcagcgtgca gctggccgac cactaccagc 5041 agaatacccc catcggcgat ggccctgtgc tgctgcccga taaccactac ctgtccaccc 5101 agagcgccct gtccaaggac cccaacgaga agcgcgatca catgatctac ttcggcttcg 5161 tgaccgccgc cgccatcacc cacggcatgg atgagctgta caagtgagcg gccgcgactc 5221 tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt 5281 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac 5341 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac 5401 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca 5461 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa 5521 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca 5581 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg 5641 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc 5701 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc 5761 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 5821 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 5881 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 5941 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 6001 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 6061 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 6121 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 6181 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 6241 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 6301 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt 6361 ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta 6421 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc 6481 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga 6541 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac 6601 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc 6661 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc 6721 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg 6781 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc 6841 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc 6901 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg 6961 aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca 7021 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca 7081 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 7141 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc 7201 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca 7261 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag 7321 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc 7381 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta 7441 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga 7501 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact 7561 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc 7621 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 7681 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 7741 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 7801 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 7861 tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat 7921 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 7981 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 8041 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 8101 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 8161 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 8221 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 8281 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 8341 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 8401 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 8461 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 8521 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 8581 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 8641 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 8701 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 8761 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 8821 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 8881 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 8941 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 9001 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 9061 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 9121 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 9181 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 9241 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 9301 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 9361 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 9421 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 9481 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 9541 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 9601 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 9661 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 9721 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 9781 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 9841 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 9901 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 9961 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 10021 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 10081 tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat 10141 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 10201 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat 10261 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta 10321 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata 10381 tgtactacaa acttagtagt //