ID pBiFC-VN173 standard; circular DNA; SYN; 5216 BP. AC .; DT 06-AUG-2018 DE Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays. KW pBiFC-VN173. OS synthetic DNA construct CC GBRFLN|REFERENCE 1 (bases 1 to 5216) CC GBRFLN| AUTHORS Shyu YJ, Liu H, Deng X, Hu CD CC GBRFLN| TITLE Identification of new fluorescent protein fragments for bimolecular CC GBRFLN| fluorescence complementation analysis under physiological CC GBRFLN| conditions. CC GBRFLN| JOURNAL Biotechniques. 2006 Jan . 40(1):61-6. CC This file is created by Vector NTI CC http://www.honorgene.com/ CC VNTAUTHORNAME | HonorGene | CC VNTAUTHORTEL | Tel：15574926881 | CC VNTAUTHOREML | Email：12695920@qq.com | FH Key Location/Qualifiers FH FT source 1..5216 FT /organism="synthetic DNA construct" FT /mol_type="other DNA" FT /vntifkey="98" FT enhancer 318..697 FT /vntifkey="9" FT /label=CMV\enhancer FT /note="human cytomegalovirus immediate early enhancer" FT promoter 698..901 FT /vntifkey="30" FT /label=CMV\promoter FT /note="human cytomegalovirus (CMV) immediate early promoter" FT CDS 935..958 FT /codon_start=1 FT /product="FLAG(R) epitope tag, followed by an enterokinase FT cleavage site" FT /vntifkey="4" FT /label=FLAG FT CDS 1028..1546 FT /codon_start=1 FT /product="N-terminal fragment of mVenus for use in FT bimolecular fluorescence complementation (BiFC) (Kodama and FT Hu, 2010)" FT /vntifkey="4" FT /label=VN173 FT polyA_signal 1557..2179 FT /vntifkey="25" FT /label=hGH\poly(A)\signal FT /note="human growth hormone polyadenylation signal" FT promoter 2208..2537 FT /vntifkey="30" FT /label=SV40\promoter FT /note="SV40 enhancer and early promoter" FT rep_origin 2388..2523 FT /vntifkey="33" FT /label=SV40\ori FT /note="SV40 origin of replication" FT rep_origin complement(3010..3598) FT /direction=LEFT FT /vntifkey="33" FT /label=ori FT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" FT CDS complement(3769..4629) FT /codon_start=1 FT /gene="bla" FT /product="beta-lactamase" FT /vntifkey="4" FT /label=AmpR FT /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" FT promoter complement(4630..4734) FT /gene="bla" FT /vntifkey="30" FT /label=AmpR\promoter FT rep_origin 4761..5216 FT /direction=RIGHT FT /vntifkey="33" FT /label=f1\ori FT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" SQ Sequence 5216 BP; 1260 A; 1370 C; 1283 G; 1303 t; ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 60 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 120 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa 180 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta 240 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 300 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg 360 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 420 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 480 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 540 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 600 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 660 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 720 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 780 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 840 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 900 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct 960 tgcggccgcg aattcatcga tagatctgat atcggtacca gtcgactcta gaagatccat 1020 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 1080 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 1140 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg 1200 gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca 1260 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 1320 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 1380 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 1440 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca 1500 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgagtagg gatcccgggt 1560 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc 1620 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct 1680 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct 1740 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca 1800 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg 1860 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt 1920 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc 1980 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt 2040 taaaataact ataccagcag gaggacgtcc agacacagca taggctacct ggccatgccc 2100 aaccggtggg acatttgagt tgcttgcttg gcactgtcct ctcatgcgtt gggtccactc 2160 agtagatgcc tgttgaattg ggtacgcggc cagcttggct gtggaatgtg tgtcagttag 2220 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 2280 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 2340 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 2400 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 2460 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 2520 gcctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt aattccctat 2580 agtgagtcgt attaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 2640 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 2700 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 2760 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 2820 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 2880 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 2940 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 3000 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 3060 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 3120 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 3180 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 3240 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 3300 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 3360 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 3420 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 3480 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 3540 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 3600 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 3660 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 3720 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 3780 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 3840 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 3900 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 3960 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 4020 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 4080 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 4140 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 4200 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 4260 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 4320 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 4380 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 4440 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 4500 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 4560 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 4620 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 4680 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 4740 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 4800 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 4860 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 4920 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 4980 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 5040 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 5100 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 5160 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caattt 5216 //