LOCUS pcDNA3.1-3xHA-C 5511 bp ds-DNA circular SYN 09-SEP-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5511) AUTHOR Name | HonorGene: http://www.honorgene.com AUTHOR Tel | 15574926881 | AUTHOR Email | 12695920@qq.com | FEATURES Location/Qualifiers source 1..5511 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 235..614 /label=CMV enhancer promoter 615..818 /label=CMV promoter CDS 997..1077 /codon_start=1 /label=3xHA /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA" polyA_signal 1106..1330 /label=BGH PolyA rep_origin 1376..1804 /direction=RIGHT /label=f1 ori promoter 1818..2147 /label=SV40 promoter CDS 2214..3008 /codon_start=1 /label=NeoR /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3184..3305 /label=SV40 PolyA rep_origin complement(3756..4344) /direction=LEFT /label=pUC ori CDS complement(4515..5375) /codon_start=1 /label=AmpR /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(5376..5480) /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca 961 gcacagtggc ggccgctcga gtctagaggg cccttctacc catatgacgt tccagactac 1021 gcgtatccgt acgacgttcc ggattacgct tacccttacg atgtacctga ctacgcttga 1081 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc 1141 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 1201 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 1261 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 1321 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg 1381 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 1441 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 1501 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 1561 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 1621 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 1681 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 1741 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 1801 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 1861 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 1921 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 1981 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 2041 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat 2101 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag 2161 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg 2221 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 2281 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 2341 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg 2401 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 2461 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 2521 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 2581 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 2641 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 2701 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg 2761 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 2821 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 2881 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 2941 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 3001 tcttctgagc gggactctgg ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca 3061 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc 3121 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac 3181 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 3241 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 3301 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag 3361 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 3421 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 3481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 3541 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 3601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 3661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 3721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 3781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 3841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 3901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 3961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 4021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 4081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 4141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 4201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 4261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 4321 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 4381 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 4441 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 4501 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 4561 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 4621 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 4681 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 4741 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 4801 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 4861 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 4921 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 4981 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 5041 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 5101 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 5161 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 5221 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 5281 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 5341 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 5401 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 5461 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c //