ID pDoubleEx-EGFP-flag-C standard; circular DNA; ; 6445 BP. DT 10-MAY-2009 CC This file is created by Vector NTI CC VNTAUTHOR Name | HonorGene: http://www.honorgene.com CC VNTAUTHOR Tel | 15574926881 | CC VNTAUTHOR Email | 12695920@qq.com | FH Key Location/Qualifiers FH FT rep_origin 5720..6363 FT /vntifkey="33" FT /label=pUC\ori FT rep_origin 3586..3816 FT /vntifkey="33" FT /label=f1\ori FT rep_origin 4157..4292 FT /vntifkey="33" FT /label=SV40\ori FT CDS 4341..5135 FT /vntifkey="4" FT /label=Kan/Neo\r FT polyA_site 2955..3179 FT /invitrogen="1870000" FT /vntifkey="26" FT /label=BGH\pA FT primer complement(2949..2966) FT /invitrogen="1910000" FT /vntifkey="27" FT /label=BGH\reverse\primer FT promoter complement(1092..2258) FT /vntifkey="29" FT /label=hEF-1¦Á\promoter FT promoter 2268..2855 FT /vntifkey="29" FT /label=CMV\promoter FT polyA_signal complement(219..321) FT /vntifkey="25" FT /label=SV40\PolyA FT CDS complement(328..1047) FT /vntifkey="4" FT /label=EGFP FT primer 2805..2825 FT /vntifkey="27" FT /label=CMV\forward\primer FT CDS 2885..2911 FT /vntifkey="4" FT /label=flag SQ Sequence 6445 BP; 1448 A; 1775 C; 1755 G; 1467 t; tagttattag ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa 60 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctttaaa 120 ggaaccaatt cagtcgataa ctataacggt cctaaggtag cgactagtcc atttaaatga 180 taacgcgtcg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 240 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 300 caatgtatct tatcatgtct gaagctttta cttgtacagc tcgtccatgc cgagagtgat 360 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt 420 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc 480 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg 540 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt 600 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc 660 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg 720 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg 780 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg 840 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt 900 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac 960 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt 1020 gaacagctcc tcgcccttgc tcaccatggt ggcagatctt cggtaccttc gaagcggccg 1080 cgaccacgtg ttcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc 1140 ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaacgagaa ttacatcaag 1200 tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa 1260 aacccctccc cccaacctaa agacgacgta ctccaaaagc tccagaacta atcgaggtgc 1320 ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc 1380 cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt 1440 gagccccctg gagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac 1500 cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga 1560 gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc 1620 cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccggccgc gggcccaaaa 1680 accgaaatac cagtgtgcag atcctggccc gcatttacaa gactatcttg ccagaaaaaa 1740 agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga 1800 caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca 1860 actcaagcac gaggcgaagg ggctcctaaa gcgcaaggcc cgcccctggc tccagctcgg 1920 gatcaagaat cacgtactgg agccaggtgg aagtaattca aggcacgcaa gggccataac 1980 ccgtaaagag gccaggcccg cgggaaccac acacggcact tacctgtgtt ctggcggcaa 2040 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac 2100 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc 2160 ttctctaggc accggttcaa ttgccgaccc ctccccccaa cttctcgggg actgtgggcg 2220 atgtgcgctc tgcccactga cgggcaccgg agcctcacga agctagcgtt gacattgatt 2280 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 2340 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2400 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2460 acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2520 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 2580 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 2640 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 2700 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 2760 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 2820 gcgtgtacgg tgggaggtct atataagcag agctcggatc ccggaattcc aactgcaggg 2880 aggtgactac aaggatgacg acgacaagtg actcgagtct agagggcccg tttaaacccg 2940 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 3000 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 3060 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 3120 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 3180 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 3240 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 3300 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 3360 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 3420 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gtctagaccc agctttcttg 3480 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact 3540 atcagtcaaa ataaaatcat tatttgccat ccagctgata tctacgtgaa ccatcaccct 3600 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 3660 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 3720 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 3780 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg 3840 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 3900 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa 3960 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 4020 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 4080 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 4140 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 4200 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 4260 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga 4320 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 4380 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 4440 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 4500 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg 4560 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 4620 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 4680 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 4740 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 4800 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 4860 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 4920 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 4980 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 5040 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 5100 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 5160 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 5220 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 5280 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac 5340 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt 5400 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc 5460 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc 5520 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc 5580 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 5640 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 5700 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 5760 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 5820 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 5880 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 5940 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 6000 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 6060 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 6120 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 6180 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 6240 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 6300 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 6360 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 6420 ggataaccgt attaccgcca tgcat 6445 //