ID pDoubleEx-EGFP standard; circular DNA; ; 6430 BP. DT 10-MAY-2009 CC This file is created by Vector NTI CC VNTAUTHOR Name | HonorGene: http://www.honorgene.com CC VNTAUTHOR Tel | 15574926881 | CC VNTAUTHOR Email | 12695920@qq.com | FH Key Location/Qualifiers FH FT rep_origin 5705..6348 FT /vntifkey="33" FT /label=pUC\ori FT rep_origin 3571..3801 FT /vntifkey="33" FT /label=f1\ori FT rep_origin 4142..4277 FT /vntifkey="33" FT /label=SV40\ori FT CDS 4326..5120 FT /vntifkey="4" FT /label=Kan+/Neo+ FT polyA_site 2940..3164 FT /invitrogen="1870000" FT /vntifkey="26" FT /label=BGH\PolyA FT primer complement(2934..2951) FT /invitrogen="1910000" FT /vntifkey="27" FT /label=BGH\reverse\primer FT promoter complement(1092..2258) FT /vntifkey="29" FT /label=hEF-1¦Á\promoter FT promoter 2268..2855 FT /vntifkey="29" FT /label=CMV\promoter FT polyA_signal complement(219..321) FT /vntifkey="25" FT /label=SV40\PolyA FT CDS complement(328..1047) FT /vntifkey="4" FT /label=EGFP FT primer 2805..2825 FT /vntifkey="27" FT /label=CMV\forward\primer SQ Sequence 6430 BP; 1439 A; 1775 C; 1750 G; 1466 t; tagttattag ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa 60 caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctttaaa 120 ggaaccaatt cagtcgataa ctataacggt cctaaggtag cgactagtcc atttaaatga 180 taacgcgtcg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 240 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 300 caatgtatct tatcatgtct gaagctttta cttgtacagc tcgtccatgc cgagagtgat 360 cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt 420 gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc 480 gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg 540 gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt 600 gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc 660 gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg 720 ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg 780 catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc tgaagcactg 840 cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt 900 gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac 960 gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt 1020 gaacagctcc tcgcccttgc tcaccatggt ggcagatctt cggtaccttc gaagcggccg 1080 cgaccacgtg ttcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc 1140 ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaacgagaa ttacatcaag 1200 tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa 1260 aacccctccc cccaacctaa agacgacgta ctccaaaagc tccagaacta atcgaggtgc 1320 ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc 1380 cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt 1440 gagccccctg gagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac 1500 cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga 1560 gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc 1620 cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccggccgc gggcccaaaa 1680 accgaaatac cagtgtgcag atcctggccc gcatttacaa gactatcttg ccagaaaaaa 1740 agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga 1800 caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca 1860 actcaagcac gaggcgaagg ggctcctaaa gcgcaaggcc cgcccctggc tccagctcgg 1920 gatcaagaat cacgtactgg agccaggtgg aagtaattca aggcacgcaa gggccataac 1980 ccgtaaagag gccaggcccg cgggaaccac acacggcact tacctgtgtt ctggcggcaa 2040 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac 2100 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc 2160 ttctctaggc accggttcaa ttgccgaccc ctccccccaa cttctcgggg actgtgggcg 2220 atgtgcgctc tgcccactga cgggcaccgg agcctcacga agctagcgtt gacattgatt 2280 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 2340 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2400 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2460 acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2520 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 2580 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 2640 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 2700 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 2760 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 2820 gcgtgtacgg tgggaggtct atataagcag agctcggatc ccggaattct gcagatatcc 2880 agcacagtgg cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac 2940 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct 3000 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct 3060 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg 3120 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag 3180 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc 3240 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 3300 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 3360 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 3420 tgattagggt gatggttcac gtagtgtcta gacccagctt tcttgtacaa agttggcatt 3480 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa 3540 atcattattt gccatccagc tgatatctac gtgaaccatc accctaatca agttttttgg 3600 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt 3660 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 3720 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 3780 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta 3840 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 3900 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa 3960 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag 4020 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag 4080 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc 4140 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 4200 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 4260 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt 4320 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 4380 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 4440 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 4500 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 4560 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 4620 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 4680 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 4740 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 4800 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 4860 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 4920 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 4980 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 5040 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 5100 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc 5160 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg 5220 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt 5280 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac 5340 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca 5400 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg 5460 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg 5520 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata 5580 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 5640 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 5700 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 5760 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 5820 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 5880 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 5940 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 6000 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 6060 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 6120 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 6180 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 6240 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 6300 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 6360 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 6420 cgccatgcat 6430 //