LOCUS pCAGGS-Flag-C 4792 bp ds-DNA circular SYN 25-OCT-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4792) AUTHOR Name | HonorGene: http://www.honorgene.com AUTHOR Tel | 15574926881 | AUTHOR Email | 12695920@qq.com | COMMENT HonorGene（奥诺基因） FEATURES Location/Qualifiers source 1..4792 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 50..73 /codon_start=1 /product="FLAG epitope tag, followed by an enterokinase cleavage site" /label=Flag /translation="DYKDDDDK" terminator 173..284 /label=rabbit globin terminator rep_origin 785..920 /label=SV40 ori /note="SV40 origin of replication" polyA_signal 940..1074 /label=SV40 PolyA /note="SV40 polyadenylation signal" rep_origin complement(1312..1900) /direction=LEFT /label=pMB1 ori /note="high-copy-number colE1/pMB1/pBR322/pUC origin of replication" CDS complement(2071..2931) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2932..3036) /gene="bla" /label=AmpR promoter enhancer 3067..3446 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 3448..3725 /label=chicken beta-actin promoter promoter 3450..4791 /label=AG promoter ORIGIN 1 aattcgagct cgcggccgcg gtaccccgac gcgtccgctc gagggaggtg attacaagga 61 tgacgacgat aagtgagcta gcagatcttt ttccctctgc caaaaattat ggggacatca 121 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag 181 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa 241 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca 301 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt 361 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt 421 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc 481 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc 541 tgcagcccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 601 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg ggtgcctaat 661 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 721 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 781 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 841 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 901 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat 961 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 1021 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc 1081 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 1141 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 1201 caaaggcggt aatacggtta tccacagaat cagggataac gcaggaaaga acatgtgagc 1261 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 1321 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 1381 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 1441 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 1501 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 1561 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 1621 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 1681 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 1741 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 1801 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 1861 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 1921 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 1981 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 2041 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 2101 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 2161 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 2221 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 2281 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 2341 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 2401 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 2461 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 2521 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 2581 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 2641 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 2701 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 2761 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 2821 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 2881 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 2941 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 3001 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 3061 tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 3121 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 3181 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 3241 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 3301 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 3361 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 3421 acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc 3481 ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg 3541 cagcgatggg ggcggggggg gggggggggc cccccccagg cggggcgggg cggggcgagg 3601 ggcggggcgg ggcgaggcgg aaaggtgcgg cggcagccaa tcagagcggc gcgctccgaa 3661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc 3721 gggcgggagt cgttgcgcgc tgccttcccc ccgtgccccg ctccgccgcc gcctcgcgcc 3781 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt 3841 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc 3901 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg 3961 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg 4021 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg 4081 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg 4141 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc 4201 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg 4261 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg 4321 gggcggggcc gcctcgggcc ggggagggct cgggggaagg ggcgcggcgg cccccggagc 4381 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag 4441 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc 4501 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc 4561 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc 4621 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg 4681 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta 4741 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa ag //