LOCUS       pNZ8149                2550 bp ds-DNA     circular SYN 11-OCT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2550)
AUTHOR Name | HonorGene: http://www.honorgene.com
AUTHOR Tel | 15574926881 |
AUTHOR Email | 12695920@qq.com |

FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        5..188
                     /label=PnisA
     primer_bind     5..24
                     /label=49-1
     terminator      393..445
                     /label=T
     CDS             788..997
                     /codon_start=1
                     /label=repC
                     /translation="MGGKEANFASVLRPPIKCRVPIFVPKTLYPNWLKGLRGFSIANES
                     PTFSPTFFINLYLSSFIFVFMITK"
     misc_feature    1266..1964
                     /label=repA
     CDS             2184..2501
                     /codon_start=1
                     /label=lacF
                     /translation="VNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVVE
                     AGSCIAEAHSSQTGMLAREASGEELPYSVTMMHGQDHLMTTILLKDVIHHLIELYKRGA
                     K"
ORIGIN
        1 ggatctagtc ttataactat actgacaata gaaacattaa caaatctaaa acagtcttaa
       61 ttctatcttg agaaagtatt ggtaataata ttattgtcga taacgcgagc ataataaacg
      121 gctctgatta aattctgaag tttgttagat acaatgattt cgttcgaagg aactacaaaa
      181 taaattataa ggaggcactc accatgggta ctgcaggcat gcggtaccac tagttctaga
      241 gagctcaagc tttctttgaa ccaaaattag aaaaccaagg cttgaaacgt tcaattgaaa
      301 tggcaattaa acaaattaca gcacgtgttg ctttgattga tagccaaaaa gcagcagttg
      361 ataaagcaat tactgatatt gctgaaaaat tgtaatttat aaataaaaat caccttttag
      421 aggtggtttt tttatttata aattattcgt ttgatttcgc tttcgataga acaatcaaat
      481 cgtttctgag acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg
      541 cgttatcgta gcgtaaaagc ccttgagcgt agcgtggctt tgcagcgaag atgttgtctg
      601 ttagattatg aaagccgatg actgaatgaa ataataagcg cagcgtcctt ctatttcggt
      661 tggaggaggc tcaagggagt ttgagggaat gaaattccct catgggtttg attttaaaaa
      721 ttgcttgcaa ttttgccgag cggtagcgct ggaaaatttt tgaaaaaaat ttggaatttg
      781 gaaaaaaatg gggggaaagg aagcgaattt tgcttccgta ctacgacccc ccattaagtg
      841 ccgagtgcca atttttgtgc caaaaacgct ctatcccaac tggctcaagg gtttgagggg
      901 tttttcaatc gccaacgaat cgccaacgtt ttcgccaacg ttttttataa atctatattt
      961 aagtagcttt atttttgttt ttatgattac aaagtgatac actaatttta taaaattatt
     1021 tgattggagt tttttaaatg gtgatttcag aatcgaaaaa aagagttatg atttctctga
     1081 caaaagagca agataaaaaa ttaacagata tggcgaaaca aaaagatttt tcaaaatctg
     1141 cggttgcggc gttagctata gaagaatatg caagaaagga atcagaacaa aaaaaataag
     1201 cgaaagctcg cgtttttaga aggatacgag ttttcgctac ttgtttttga taaggtaatt
     1261 atatcatggc tattaaaaat actaaagcta gaaattttgg atttttatta tatcctgact
     1321 caattcctaa tgattggaaa gaaaaattag agagtttggg cgtatctatg gctgtcagtc
     1381 ctttacacga tatggacgaa aaaaaagata aagatacatg gaatagtagt gatgttatac
     1441 gaaatggaaa gcactataaa aaaccacact atcacgttat atatattgca cgaaatcctg
     1501 taacaataga aagcgttagg aacaagatta agcgaaaatt ggggaatagt tcagttgctc
     1561 atgttgagat acttgattat atcaaaggtt catatgaata tttgactcat gaatcaaagg
     1621 acgctattgc taagaataaa catatatacg acaaaaaaga tattttgaac attaatgatt
     1681 ttgatattga ccgctatata acacttgatg aaagccaaaa aagagaattg aagaatttac
     1741 ttttagatat agtggatgac tataatttgg taaatacaaa agatttaatg gcttttattc
     1801 gccttagggg agcggagttt ggaattttaa atacgaatga tgtaaaagat attgtttcaa
     1861 caaactctag cgcctttaga ttatggtttg agggcaatta tcagtgtgga tatagagcaa
     1921 gttatgcaaa ggttcttgat gctgaaacgg gggaaataaa atgacaaaca aagaaaaaga
     1981 gttatttgct gaaaatgagg aattaaaaaa agaaattaag gacttaaaag agcgtattga
     2041 aagatacaga gaaatggaag ttgaattaag tacaacaata gatttattga gaggagggat
     2101 tattgaataa ataaaagccc ccctgacgaa agtcgacatg gactgataaa gtatagtaaa
     2161 aacataaaac ggaggatatt gttgtgaaca gagaagagat gactctctta gggtttgaaa
     2221 ttgttgctta tgctggagat gctcgctcta agcttttaga agcgcttaaa gcggctgaaa
     2281 atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt attgcagagg
     2341 ctcacagttc tcagacaggt atgttggctc gagaagcttc tggggaggaa cttccataca
     2401 gtgttactat gatgcatggt caggatcact tgatgactac gatcttatta aaagatgtga
     2461 ttcatcacct catcgaactt tataaaagag gagcaaagta attaatgcat aaactcattg
     2521 aacttattga gaaagggaaa cgacggatca
//