ID pGFPuv standard; circular DNA; SYN; 3337 BP. DT 19-DEC-2013 CC Created by HonorGene：http://www.honorgene.com CC This file is created by Vector NTI CC ORIGDB|GenBank CC VNTDBDATE|-12412332| CC LSOWNER| CC VNTAUTHOR NAME| HonorGene | http://www.honorgene.com CC VNTAUTHOR TEL|15574926881| CC VNTAUTHOR EML|12695920@qq.com| FH Key Location/Qualifiers FH FT misc_feature 95..178 FT /vntifkey="21" FT /label=lac\promoter FT misc_feature 234..281 FT /vntifkey="21" FT /label=5'\MCS FT CDS 289..1005 FT /vntifkey="4" FT /label=GFPuv FT /note="GFP variant optimized for excitation by UV light" FT misc_feature 1007..1091 FT /vntifkey="21" FT /label=3'\MCS FT promoter 1432..1536 FT /vntifkey="30" FT /label=AmpR\promoter FT CDS 1537..2397 FT /vntifkey="4" FT /label=Amp+ FT misc_feature 2545..3188 FT /vntifkey="21" FT /label=pUC\ori FT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" SQ Sequence 3337 BP; 886 A; 785 C; 823 G; 843 t; agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 240 catgcctgca ggtcgactct agaggatccc cgggtaccgg tagaaaaaat gagtaaagga 300 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg 360 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt 420 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc 480 tcttatggtg ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc 540 aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg 600 aactacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag 660 ttaaaaggta ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac 720 tataactcac acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac 780 ttcaaaattc gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa 840 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa 900 tctgcccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta 960 actgctgctg ggattacaca tggcatggat gagctctaca aataatgaat tccaactgag 1020 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggcct actagtcggc 1080 cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 1140 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 1200 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 1260 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 1320 aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata ggttaatgtc 1380 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 1440 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 1500 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 1560 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 1620 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 1680 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 1740 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa 1800 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 1860 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 1920 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 1980 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 2040 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg 2100 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 2160 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 2220 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 2280 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 2340 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 2400 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 2460 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 2520 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 2580 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 2640 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 2700 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 2760 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 2820 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 2880 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 2940 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 3000 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 3060 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 3120 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 3180 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 3240 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 3300 accgagcgca gcgagtcagt gagcgaggaa gcggaag 3337 //