LOCUS Exported 5672 bp ds-DNA circular SYN 04-SEP-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled 4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5672) AUTHORS . TITLE Direct Submission JOURNAL Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4 http://www.yrgene.com FEATURES Location/Qualifiers source 1..5672 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 109..140 /note="minP" /note="minimal TATA-box promoter with low basal activity" CDS 173..1825 /codon_start=1 /product="firefly luciferase" /note="luciferase" /note="synthetic luc2 version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" polyA_signal 1869..1990 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" promoter 2184..2541 /note="SV40 promoter" /note="SV40 enhancer and early promoter" rep_origin 2392..2527 /note="SV40 ori" /note="SV40 origin of replication" CDS 2572..3366 /codon_start=1 /product="aminoglycoside phosphotransferase" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3391..3439 /note="synthetic polyadenylation signal" rep_origin complement(3766..4354) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(4554..5414) /codon_start=1 /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" polyA_signal 5519..5567 /note="synthetic polyadenylation signal" misc_feature 5581..5672 /note="pause site" /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" ORIGIN 1 ggcctaactg gccggtaccg ctagcctcga gtgcttcccg aacgttgctt cccgaacgtt 61 gcttcccgaa cgttgcttcc gaacgtagat ctgcagaagc ttagacacta gagggtatat 121 aatggaagct cgacttccag cttggcaatc cggtactgtt ggtaaagcca ccatggaaga 181 tgccaaaaac attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg 241 cgagcagctg cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac 301 cgacgcacat atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct 361 ggcagaagct atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga 421 gaatagcttg cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc 481 cccagctaac gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc 541 caccgtcgta ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct 601 accgatcata caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag 661 catgtacacc ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc 721 cgagagcttc gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg 781 attgcccaag ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg 841 cgaccccatc ttcggcaacc agatcatccc cgacaccgct atcctcagcg tggtgccatt 901 tcaccacggc ttcggcatgt tcaccacgct gggctacttg atctgcggct ttcgggtcgt 961 gctcatgtac cgcttcgagg aggagctatt cttgcgcagc ttgcaagact ataagattca 1021 atctgccctg ctggtgccca cactatttag cttcttcgct aagagcactc tcatcgacaa 1081 gtacgaccta agcaacttgc acgagatcgc cagcggcggg gcgccgctca gcaaggaggt 1141 aggtgaggcc gtggccaaac gcttccacct accaggcatc cgccagggct acggcctgac 1201 agaaacaacc agcgccattc tgatcacccc cgaaggggac gacaagcctg gcgcagtagg 1261 caaggtggtg cccttcttcg aggctaaggt ggtggacttg gacaccggta agacactggg 1321 tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg atcatgagcg gctacgttaa 1381 caaccccgag gctacaaacg ctctcatcga caaggacggc tggctgcaca gcggcgacat 1441 cgcctactgg gacgaggacg agcacttctt catcgtggac cggctgaaga gcctgatcaa 1501 atacaagggc taccaggtag ccccagccga actggagagc atcctgctgc aacaccccaa 1561 catcttcgac gccggggtcg ccggcctgcc cgacgacgat gccggcgagc tgcccgccgc 1621 agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag gagatcgtgg actatgtggc 1681 cagccaggtt acaaccgcca agaagctgcg cggtggtgtt gtgttcgtgg acgaggtgcc 1741 taaaggactg accggcaagt tggacgcccg caagatccgc gagattctca ttaaggccaa 1801 gaagggcggc aagatcgccg tgtaataatt ctagagtcgg ggcggccggc cgcttcgagc 1861 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 1921 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 1981 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 2041 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtt 2101 tgcgtattgg gcgctcttcc gctgatctgc gcagcaccat ggcctgaaat aacctctgaa 2161 agaggaactt ggttagctac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag 2221 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 2281 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa 2341 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc 2401 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 2461 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt 2521 ggaggcctag gcttttgcaa aaagctcgat tcttctgaca ctagcgccac catgatcgaa 2581 caagacggcc tccatgctgg cagtcccgca gcttgggtcg aacgcttgtt cgggtacgac 2641 tgggcccagc agaccatcgg atgtagcgat gcggccgtgt tccgtctaag cgctcaaggc 2701 cggcccgtgc tgttcgtgaa gaccgacctg agcggcgccc tgaacgagct tcaagacgag 2761 gctgcccgcc tgagctggct ggccaccacc ggcgtaccct gcgccgctgt gttggatgtt 2821 gtgaccgaag ccggccggga ctggctgctg ctgggcgagg tccctggcca ggatctgctg 2881 agcagccacc ttgcccccgc tgagaaggtt tctatcatgg ccgatgcaat gcggcgcctg 2941 cacaccctgg accccgctac ctgccccttc gaccaccagg ctaagcatcg gatcgagcgt 3001 gctcggaccc gcatggaggc cggcctggtg gaccaggacg acctggacga ggagcatcag 3061 ggcctggccc ccgctgaact gttcgcccga ctgaaagccc gcatgccgga cggtgaggac 3121 ctggttgtca cacacggaga tgcctgcctc cctaacatca tggtcgagaa tggccgcttc 3181 tccggcttca tcgactgcgg tcgcctagga gttgccgacc gctaccagga catcgccctg 3241 gccacccgcg acatcgctga ggagcttggc ggcgagtggg ccgaccgctt cttagtcttg 3301 tacggcatcg cagctcccga cagccagcgc atcgccttct accgcttgct cgacgagttc 3361 ttttaatgat ctagaaccgg tcatggccgc aataaaatat ctttattttc attacatctg 3421 tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac cgatgccctt gagagccttc 3481 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact 3541 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct 3601 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 3661 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3721 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 3781 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3841 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 3901 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3961 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 4021 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 4081 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 4141 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 4201 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 4261 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 4321 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 4381 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 4441 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 4501 atatgagtaa acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag 4561 tgcttgatca gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc 4621 tgactccccg tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca 4681 gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca 4741 gcagggaggg ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg 4801 agctgctgtc gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg 4861 gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct 4921 ggttcccagc ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc 4981 tccttagggc ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta 5041 atggcagcac tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc 5101 ggcgagtact caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc 5161 ccggcgtcta tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc 5221 gggaatcgtt cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg 5281 atatagccca ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg 5341 gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa 5401 tgttggatgc tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag 5461 tacgtctctc aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa 5521 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact 5581 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc 5641 cagtgcaagt gcaggtgcca gaacatttct ct //