pGBKT7-53

载体特点：与pGADT7-T构成Matchmaker Two-Hybrid System 3的双杂交正对照。

pGBKT7-53 encodes a fusion of the murine p53 protein (a.a. 72–390) and the GAL4 DNA-BD (a.a. 1–147). The murine p53 cDNA (GenBank Accession #K01700) was cloned into pGBKT7 at the EcoR I and BamH I sites. The p53 insert was derived from the plasmid described in Iwabuchi et al. (1993); plasmid modification was performed at Clontech. pGBKT7-53 has not been sequenced.

pGBKT7-53 载体说明(点击下载)