pLNHX

pLNHX contains elements derived from Moloney murine leukemia virus (MoMuLV) and Moloney murine sarcoma virus (MoMuSV), and is designed for retroviral gene delivery and expression (1–4). Upon transfection into a packaging cell line, pLNHX can transiently express, or integrate and stably express, a transcript containing the viral packaging signal Ψ+, the neomycin selection marker (Neor), and a target gene. The 5' viral LTR in this vector contains promoter/enhancer sequences that control expression of the Neor gene, which allows antibiotic selection in eukaryotic cells. A target gene can be cloned into the multiple cloning site downstream of the Drosophila heat shock protein 70 (hsp70) promoter (Phsp70). pLNHX also includes the Col E1 origin of replication and E. coli Ampr gene for propagation and antibiotic selection in bacteria.

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