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pTet-On

产品货号:HG-VSC0460
载体名称:pTet-On
出品公司:Clontech
载体用途:蛋白表达
出货周期:现货
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产品概述
产品名称pTet-On产品货号HG-VSC0460
载体名称pTet-On出货周期现货
载体介绍

pTet-On expresses the reverse tet-responsive transcriptional activator (rtTA) from the strong immediate early promoter of cytomegalovirus (PCMV). tTA is a fusion of amino acids 1–207 of the tet repressor (TetR) and the negatively charged C-terminal activation domain (130 amino acids) of the VP16 protein of herpes simplex virus. pTet-On is similar to pTet-Off except for four amino acid changes that convert the TetR to a rTetR, and consequently, tTA to rtTA. (pTet-Off also contains several silent mutations.) pTet-On was originally described as pUHD17-1neo by Gossen et al. pTet-On can be distinguished from pTet-Off by digestion with Hind III.

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质粒图谱