pFC-MEKK

Positive control plasmid. In the trans-reporting system use with the c-Jun reporting system. In the cis-reporting system use with pNF-kB-Luc, pNF-kB-hrGFP, pAP1-Luc, pAP1-hrGFP, and pSRE-Luc plasmids.

The PathDetect Signal Transduction Pathway cis-Reporting Systems are designed for simple, rapid, and convenient assessment of the in vivo activation of signal transduction pathways. There are a series of inducible reporter plasmids that contain the luciferase reporter gene driven by a basic promoter element (TATA box) plus a defined inducible cis-enhancer element.

•  Identify pathway-specific transcription activation

•  Easier, faster than Western blotting or gel-shift assays

•  Sensitive, quantitative assays

•  Antibodies now available

•  Stable cell lines available for constitutive expression of CREB, Elk1, c-Jun, and CHOP

•  PathDetect Trans-Reporting Systems -Identify specific stimuli that leads to the phosphorylation of a test transcription factor