pET-17b(+)

The pET-17b vector carries an N-terminal 11aa T7 tag sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker. Unique sites (except for the two BstX I sites) are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circluar map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.

pET-17b(+) 载体说明(点击下载)

pET-17b(+) 载体序列(点击下载)