pRSFDuet-1

pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the RSF1030-derived RSF replicon, lacI gene and kanamycin resistance gene (KnR). This vector can be used in combination with pACYCDuet™-1, pCDFDuet™-1, and pETDuet™-1 in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. Unique sites are shown on the circle map.

pRSFDuet-1产品说明书.pdf

pRSFDuet-1产品手册（Novagen）.pdf

pRSFDuet-1质粒序列.txt