pGL3-U6-sgRNA-PGK-puromycin

Expresses sgRNA from the U6 promoter.

相关参考文献：

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Iyer V, Huang X, Skarnes WC. Nat Methods. 2014 Mar 2. 

PubMed 24584192：

https://www.ncbi.nlm.nih.gov/pubmed/24584192

pGL3-U6-sgRNA-PGK-puromycin(点击下载)

pGL3-U6-sgRNA-PGK-puromycin质粒序列.txt