pFN21A

The pFN21A and pFN21K HaloTag® CMV Flexi® Vectors are configured to append HaloTag® protein tag to the aminoterminus of the protein fusion partner and provide constitutive protein expression in mammalian cells using the human cytomegalovirus (CMV) immediate-early enhancer/promoter. The vectors can be used for both stable and transient gene expression; for stable expression, cotransfection with a vector containing a selectable marker is required. The vectors are provided with a choice of ampicillin (A) or kanamycin (K) antibiotic resistance genes.

Note: Flexi® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coliwithout an insert.

Application: Clone ORF and conveniently transfer these inserts to a variety of vectors designed for multiple applications.