产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
---|---|---|---|---|---|
HG-VJI0902 | pYES2/CT | Invitrogen |
酵母表达载体
|
询价 | 现货 |
质粒类型:酵母表达载体,酿酒酵母表达载体
载体大小:5963bp
载体抗性:Ampicillin (氨苄青霉素)
筛选标记:URA3
载体标签:C-His, C-V5
克隆方法:多克隆位点,限制性内切酶
启动子:GAL1 promoter
表达水平:高
诱导方法:半乳糖
5' 测序引物:GAL1-F(5'-AATATACCTCTATACTTTAACGTC-3')
3' 测序引物:CYC1-R(5'-GCGTGAATGTAAGCGTGAC-3')
备注:利用半乳糖诱导蛋白在酿酒酵母中表达。
pYES2/NT and pYES2/CT are S. cerevisiae expression vectors derived from the parental pYES2 vector. Both vectors offer the URA3 gene for selection in yeast. pYES2/NT features an N-terminal Xpress™ epitope for detection with Invitrogen's Anti-Xpress™ Antibody and a polyhistidine (6xHis) tag for purification with nickel-chelating resin. pYES2/CT contains a C-terminal V5 epitope for detection and a polyhistidine (6xHis) tag for purification.