质粒类型：慢病毒载体

高拷贝/低拷贝：高拷贝

启动子：CMV

克隆方法：多克隆位点，限制性内切酶

载体大小：8778 bp 

5' 测序引物及序列：CMV-F:CGCAAATGGGCGGTAGGCGTG(Invitrogen)

载体标签：N-mCherry

载体抗性：Ampicillin (氨苄青霉素)

筛选标记：Puromycin (嘌呤霉素)

备注：载体能够表达N端mCherry荧光蛋白。

pLVX-mCherry-C1 is an HIV-1-based, lentiviral expression vector that allows you to express your gene of interest fused to mCherry, a mutant fluorescent protein derived from the tetrameric Discosoma sp. red fluorescent protein, DsRed (1). The excitation and emission maxima of the native mCherry protein are 587 nm and 610 nm, respectively. Genes cloned into the multiple cloning site (MCS), located at the C-terminal end of the mCherry coding sequence, are expressed as C-terminal mCherry fusion proteins. Expression of the fusion protein is driven by the constitutively active human cytomegalovirus immediate early promoter (PCMV IE), located just upstream of the mCherry coding sequence. Lentiviral particles derived from the vector allow the expression of mCherry fusion proteins in virtually any cell type, including primary cells. The unmodified vector expresses mCherry, and may be used to produce marker virus to optimize infection protocols.

pLVX-mCherry-C1 contains all of the viral processing elements necessary for the production of replication-incompetent lentivirus, as well as elements to improve viral titer, transgene expression, and overall vector function. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) promotes RNA processing events and enhances nuclear export of viral and transgene RNA (2), leading to increased viral titers from packaging cells, and enhanced expression of your gene of interest in target cells. In addition, the vector includes a Rev-response element (RRE), which further increases viral titers by enhancing the transport of unspliced viral RNA out of the nucleus (3). Finally, pLVX-mCherry-C1 also contains a central polypurine tract/central termination sequence element (cPPT/CTS). During target cell infection, this element creates a central DNA flap that increases nuclear import of the viral genome, resulting in improved vector integration and more efficient transduction (4).

In addition to lentiviral elements, pLVX-mCherry-C1 contains a puromycin resistance gene (Puror) under the control of the murine phosphoglycerate kinase (PGK) promoter (PPGK) for the selection of stable transductants. The vector also contains a pUC origin of replication and an E. coli ampicillin resistance gene (Ampr) for propagation and selection in bacteria.

pLVX-mCherry-C1产品说明书.pdf

pLVX-mCherry-C1质粒序列.txt