| 产品货号 | 载体名称 | 出品公司 | 载体用途 | 产品价格 | 出货周期 |
|---|---|---|---|---|---|
| HG-VYN0195 | pETDuet-1 | Novagen |
原核表达载体
|
询价 | 现货 |
质粒类型：大肠杆菌表达载体（双启动子）
表达水平：高
克隆方法：多克隆位点，限制性内切酶
载体大小：5420 bp
5' 测序引物：T7
3' 测序引物：T7-ter
载体标签：N-His, C-S
载体抗性：Ampicillin (氨苄青霉素)
备注：pETDuet-1 is designed for the coexpression of two target genes. pETDuet-1 carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene.
pETDuet-1 is designed for the coexpression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and a ribosome binding site (rbs). The vector also carries the pBR322-derived ColE1 replicon, lacI gene and ampicillin resistance gene. This vector can be used in combination with pACYCDuet™-1 in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the pET Upstream Primer and DuetDOWN1 Primer. Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.
